Hide
Show all
Email this article Application Technique and Dosage of Halotolerant Nitrogen Biofertilizer for Increasing Soil Total N, N Uptake, Chlorophyll Content, Photosynthate Accumulation and Growth of Rice Plants in Saline Ecosystem
Abstract
Utilization of halotolerant nitrogen biofertilizer can increase N uptake and promote growth and yield of rice plants in saline ecosystem. Halotolerant nitrogen biofertilizer can be applied to seed and seedling in certain dosage. The aim of this study was to obtain the application technique and dosage that can increase N uptake and promote growth and yield of rice plants in saline ecosystem. The research was conducted in the greenhouse in Kawarang District (Indonesia) from September until November 2020. There was used randomized block design which consisted of two factors with three replications. The first factor was application techniques, i.e. seed treatment, nursery treatment, and seed + nursery treatment. The second factor was dosage of halotolerant nitrogen biofertilizer, i.e. 0, 500, 1,000, 1,500 and 2,000 g ha-1. The results showed that halotolerant nitrogen biofertilizer applied in seed and nursery treatment can increase N uptake and plant height by 3.95 g plant-1 and 34.50 cm, respectively. Dosage of 1,500 g ha-1 can increase the total N of soil (0.26%), chlorophyll content (46.97 SPAD), photosynthate accumulation (3.33 g), and rice yield (13.40 t/ha). Application of halotolerant nitrogen biofertilizer in seed and nursery technique at a dosage of 1,500 g ha-1 can be further recommendation in rice cultivation on saline ecosystems.
Keywords
Full Text:
PDFReferences
Aon, M., Khalid, M., Hussain, S., Naveed, M., Akhtar, M.J., 2015. Diazotrophic inoculation supplemented nitrogen demand of flooded rice under field conditions. Pakistan Journal of Agricultural Sciences, 52(1), 145–150.
Arifin, M.S., Rion, M.S.I., Rahman, A., Zakir, H.M., Quadir, Q.F., 2021. Salt tolerant rhizobacteria from coastal region of Bangladesh portrayed the potential for plant growth promotion. Journal of Applied Life Sciences International, 24: 58–70.
Balkan, A., Gençtan, T., Bilgin, O., Ulukan, H., 2015. Response of rice (Oryza sativa L.) to salinity stress at germination and early seedling stages. Pakistan Journal of Agricultural Sciences, 52(2): 455–461.
Bano, A., Fatima, M., 2009. Salt tolerance in Zea mays (L) following inoculation with Rhizobium and Pseudomonas. Biology and Fertility of Soils, 45(4): 405–413.
Basu, A., Prasad, P., Das, S.N., Kalam, S., Sayyed, R.Z., Reddy, M.S., El Enshasy, H., 2021. Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: Recent developments, constraints, and prospects. Sustainability, 13(3): 1140.
Biswas, J.C., Ladha, J.K., Dazzo, F.B., 2000. Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Soil Science Society of America Journal, 64(5): 1644–1650.
Bremner, J.M., 1965. Total nitrogen. Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, 9: 1149–1178. https://doi.org/10.2134/agronmonogr9.2.c32.
Chauhan, H., Bagyaraj, D.J., Sharma, A., 2013. Plant growth-promoting bacterial endophytes from sugarcane and their potential in promoting growth of the host under field conditions. Experimental Agriculture, 49(1): 43.
Chen, P., Liu, Y., Mo, C., Jiang, Z., Yang, J., Lin, J., 2021. Microbial mechanism of biochar addition on nitrogen leaching and retention in tea soils from different plantation ages. Science of The Total Environment, 757: 143817.
de Mesquita Alves, J., de Lima, A.S., de Mesquita, E.F., Junior, S.D.O.M., de Sousa Ferreira, R., da Silva, F.L., da Mota Santos, J., 2018. Gas exchange and chlorophyll content in tomato grown under different organic fertilizers and biofertilizer doses. African Journal of Agricultural Research, 13(41): 2256–2262.
Doni, F., Suhaimi, N.S.M., Irawan, B., Mohamed, Z., Mispan, M.S., 2021. Associations of Pantoea with rice plants: As friends or foes? Agriculture, 11(12): 1278.
Etesami, H., Mirsyed Hosseini, H., Alikhani, H.A., 2014. In planta selection of plant growth promoting endophytic bacteria for rice (Oryza sativa L.). Journal of Soil Science and Plant Nutrition, 14(2): 491–503.
FAO, 1988. Salt-Affected Soils and their Management.
Hussain, M., Ahmad, S., Hussain, S., Lal, R., Ul-Allah, S., Nawaz, A., 2018. Rice in saline soils: Physiology, biochemistry, genetics, and management. Advances in Agronomy, 148: 231–287.
Ivanova, K., Anev, S., Tzvetkova, N., Georgieva, T., Markovska, Y., 2015. Influence of salt stress on stomatal, biochemical and morphological factors limiting photosynthetic gas exchange in Paulownia elongata × fortunei. Comptes rendus de l’Académie bulgare des Sciences, 68(2): 217–224.
Khumairah, F.H., Azwari, F., Setiawati, M.R., Fitriatin, B.N., Simarmata, T., 2022a. Effect of different carrier composition on viability of halotolerant n-fixing rhizobacteria, water content and pH level in nitrogen biofertilizer for saline ecosystem. Kultivasi, 21(1).
Khumairah, F.H., Setiawati, M.R., Fitriatin, B.N., Simarmata, T., Alfarraj, S., Ansari, M.J., El Enshasy, H.A., Sayyed, R.Z., 2022b. Halotolerant plant growth promoting rhizobacteria isolated from saline soil improve nitrogen fixation and alleviate salt stress in rice plants. Frontiers in Microbiology, 1607.
Khumairah, F.H., Ulfah, T., Setiawati, M.R., Fitriatin, B.N., Simarmata, T., 2022c. Population and biochemical characteristics of halotolerant N2 fixing rhizobacteria isolated from saline soil ecosystem. Jurnal AgroBiogen, 18(1): 1–10.
Larimi, S.B., Shakiba, M., Mohammadinasab, A.D., Vahed, M.M., 2014. Changes in nitrogen and chlorophyll density and leaf area of sweet basil (Ocimum basilicum L.) affected by biofertilizer and nitrogen application. International Journal of Biosciences, 5(9): 256–265.
Li, Y., Li, H., Han, X., Han, G., Xi, J., Liu, Y., Zhang, Y., Xue, Q., Guo, Q., Lai, H., 2022. Actinobacterial biofertilizer improves the yields of different plants and alters the assembly processes of rhizosphere microbial communities. Applied Soil Ecology, 171: 104345.
Lincoln, T., Edvardo, Z., 2006. Assimilation of Mineral Nutrition. Plant Physiology (4th ed.). Sinauer Associates, Sunderland MA.
Lisle, L., Gaudron, J., Lefroy, R., 1990. Laboratory Techniques for Plant and Soil Analysis. UNE-ACIAR-Crawford Fund. Department of Agronomy and Soil Science, University of New England, Armidale. Australian Centre for International Agricultural Research.
Mącik, M., Gryta, A., Frąc, M., 2020. Biofertilizers in agriculture: An overview on concepts, strategies and effects on soil microorganisms. Advances in Agronomy, 162: 31–87.
Munns, R., 2002. Comparative physiology of salt and water stress. Plant, Cell & Environment, 25(2): 239–250.
Munns, R., Tester, M., 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59: 651–681.
Mutalib, A.A., Radziah, O., Shukor, Y., Naher, U.A., 2012. Effect of nitrogen fertilizer on hydrolytic enzyme production, root colonisation, N metabolism, leaf physiology and growth of rice inoculated with ‘Bacillus’ sp. (Sb42). Australian Journal of Crop Science, 6(9): 1383–1389.
Netondo, G.W., Onyango, J.C., Beck, E., 2004. Sorghum and salinity: II. Gas exchange and chlorophyll fluorescence of sorghum under salt stress. Crop Science, 44(3): 806–811.
Oren, A., Rodríguez-Valera, F., 2001. The contribution of halophilic bacteria to the red coloration of saltern crystallizer ponds. FEMS Microbiology Ecology, 36(2–3): 123–130.
Rachman, A., Dariah, A., Sutono, S., 2018. Management of High Saline Rice Fields. IAARD Press, Jakarta.
Rahman, K.M., Zhang, D., 2018. Effects of fertilizer broadcasting on the excessive use of inorganic fertilizers and environmental sustainability. Sustainability, 10(3): 759.
Setiawati, M.R., Al-Azmiya, N.U., Khumairah, F.H., Simarmata, T., 2022. Halotolerant n-fixing bacteria isolates for increasing the biochemical activity, total bacteria population, N-uptake and rice seedling growth. KnE Life Sciences, 1–12.
Shrivastava, P., Kumar, R., 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22(2): 123–131.
Syed, A., Sarwar, G., Shah, S.H., Muhammad, S., 2021. Soil salinity research in the 21st century in Pakistan: Its impact on availability of plant nutrients, growth and yield of crops. Communications in Soil Science and Plant Analysis, 52(3): 183–200.
van Reeuwijk, L.P., 1993. Procedures for Soil Analysis (4th ed.). Technical Paper, International Soil Reference and Information Centre, Wageningen.
Wakelin, S.A., Gupta, V.V.S.R., Forrester, S.T., 2010. Regional and local factors affecting diversity, abundance and activity of free-living, N2-fixing bacteria in Australian agricultural soils. Pedobiologia, 53(6): 391–399.
Wang, C.F., Han, G.L., Yang, Z.R., Li, Y.X., Wang, B.S., 2022. Plant salinity sensors: Current understanding and future directions. Frontiers in Plant Science, 13.
Yanni, Y.G., Rizk, R.Y., Abd El-Fattah, F.K., Squartini, A., Corich, V., Giacomini, A., de Bruijn, F., Rademaker, J., Maya-Flores, J., Ostrom, P., Vega-Hernandez, M., 2001. The beneficial plant growth-promoting association of Rhizobium leguminosarum bv. trifolii with rice roots. Functional Plant Biology, 28(9): 845–870.
Zhang, W., Wang, C., Lu, T., Zheng, Y., 2018. Cooperation between arbuscular mycorrhizal fungi and earthworms promotes the physiological adaptation of maize under a high salt stress. Plant and Soil, 423(1): 125–140.
Zhou, Jing., Guiyuan, M.E.N.G., Chenzhong, J.I.N., Guohui, M.A., Jirui, L.O.N.G., 2015. Effects of nitrogen rate on blast resistance and grain yield of super early hybrid rice Luliangyou 996. Agricultural Science & Technology, 16(9).
DOI: http://dx.doi.org/10.17951/pjss.2022.55.2.79-91
Date of publication: 2022-12-29 11:25:05
Date of submission: 2021-04-22 06:57:49
Statistics
Indicators
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Mieke Rochimi Setiawati, Fiqriah Hanum Khumairah, Betty Natalie Fitriatin, Tualar Simarmata
This work is licensed under a Creative Commons Attribution 4.0 International License.